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Biotechnology

Biotechnology

We use recombinant DNA technology and synthetic chemistry to develop proteins and peptides with applications in research, therapy, diagnostic and bioprocessing. Here is a general flow chart of our areas of actuation.

Analysis & Production

Peptides

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Proteins

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- Determine the structural identity and stability

- Use scalable recombinant production protocols (milligrams)

- Solid-phase synthesis (micrograms)

Diagnostics

Development and assessment of a multiepitope synthetic antigen for the diagnosis of Dengue virus infection.

Da Silva, I.B.N. et al 

Brazilian Journal of Infectious Diseases, 2024.

Accuracy of a raw saliva-based COVID-19 RT-LAMP diagnostic assay

Cabral K.M.S., et al. 

Brazilian Journal of Infectious Diseases, 2023.

Therapy

Formation of subvisible particles in commercial insulin formulations.

SILVA-JR, HAMILTON ; ARAÚJO, TALITA S. ; DA SILVA ALMEIDA, MARCIUS ; SCAPIN, SANDRA M.N. ; LUÍS MAURÍCIO, T.R.

COLLOIDS AND SURFACES B-BIOINTERFACES, v. 216, 2022.

Unambiguous characterization of PEGylation site on human amylin by two‐dimensional nuclear magnetic resonance spectroscopy

LIMA, LUÍS MAURÍCIO TR; DE ARAÚJO, TALITA STELLING; ALMEIDA, MARCIUS S. PEPTIDE SCIENCE, v. 113, 2021.

Biophysical characterization of two commercially available preparations of the drug containing Escherichia coli L-Asparaginase 2.

DE ARAÚJO, TALITA STELLING; SCAPIN, SANDRA MN; DE ANDRADE, WILLIAM; FASCIOTTI, MAÍRA; DE MAGALHÃES, MARIANA TQ; ALMEIDA, MARCIUS S; LIMA, LUÍS MAURÍCIO TR. BIOPHYSICAL CHEMISTRY, v. 1, p. 106554, 2021.

Monitoring asparaginase activity.

LIMA, LUÍS MAURÍCIO T R; ARAUJO, TALITA S; ALMEIDA, FÁBIO C L; ALMEIDA, MARCIUS S.

LANCET ONCOLOGY, v. 19, p. e574, 2018.

Ligand-free method to produce the anti-angiogenic recombinant Galectin-3 carbohydrate recognition domain.

WIECIKOWSKI, ADALGISA ; CABRAL, KATIA MARIA DOS SANTOS ; DA SILVA ALMEIDA, MARCIUS ; CARVALHO, RENATO SAMPAIO.

PROTEIN EXPRESSION AND PURIFICATION, v. 1, p. 1-2, 2017.

Bioprocessing

Properties of novel surfactin-derived biosurfactants obtained through solid-phase synthesis.

VAZQUEZ, LEONARDO ; TEIXEIRA DA SILVA FERREIRA, ANDRE ; CAVALCANTE, FERNANDA SAMPAIO ; GARCIA, ISRAEL JOSÉ P. ; DOS SANTOS, KATIA REGINA NETTO ; BARBOSA, LEANDRO AUGUSTO DE OLIVEIRA ; Almeida, Marcius da Silva ; MIGNACO, JULIO ALBERTO ; FONTES, CARLOS FREDERICO LEITE.

JOURNAL OF PEPTIDE SCIENCE, v. 1, p. e3129, 2018.

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